A handheld sensor assay for the identification of grouper as a safeguard against seafood mislabeling fraud

Increases in international trade and global seafood consumption, along with fluctuations in the supply of different seafood species, have resulted in fraudulent product mislabeling. Grouper species, due to their high demand and varied commercial availability, are common targets for mislabeling by exploiting inefficient inspection practices. Compounding this problem is the fact that there are currently 64 species of fish from eleven different genera allowed to be labeled “grouper” per U.S. Food and Drug Administration guidelines. This wide diversity makes it difficult for regulators to discern legally salable groupers from restricted species. To obviate taxonomic misidentification when relying on external phenotypic characteristics, regulatory agencies are now employing genetic authentication methods which typically offer species-level resolution. However, standard genetic methods such as DNA barcoding require technical expertise and long turnover times, and the required instrumentation is not amenable for on-site analysis of seafood. To obviate some of these limitations, we have developed a handheld genetic sensor that employs a real-time nucleic acid sequence-based amplification assay (RT-NASBA) previously devised in our lab, for the analysis of fish tissue in the field. The base RT-NASBA assay was validated using a lab-based, benchtop RNA purification method as well as non-portable, commercial RT-NASBA analyzer. Described herein, is an uncomplicated method for purifying RNA from fish tissue in the field, which had similar efficiency to the benchtop method demonstrated through direct comparisons. We have also demonstrated that the field sensor is only slightly less sensitive than the benchtop instrument, and could discern 80.3% of groupers (no target sequence available for three species) on the 2014 FDA Seafood List from potential impostors. The complete field assay requires fewer than 80 min for completion and can be performed outside of the lab in its entirety.     

How a knowledge-based approach might illuminate the notion of human capital and its measurement

Human capital measurement and accounting has been under discussion for years without any satisfactory methodology emerging. The economic significance of today's knowledge-intensive organizations makes better HC measurement more pressing. We draw on insights from the knowledge-based theory of the firm and conclude we can only make sense of its human capital by looking in detail at its practices. Human capital is the value added at the level of the work practice—as traced by activity-base accounting. Overall the firm's human capital totals into its goodwill. The human capital can be estimated and then managed by allocating the goodwill to the activities taking place, a complex distribution process but one precisely complementary to that of activity-base accounting.     

Competency or incompetency--the dilemma

RATIONALE AND OBJECTIVES: Simplifying data collection and analysis should promote utilization management in review of examinations of the small bowel in the general practice. METHODS: A case control format with the generation of an odds ratio to answer sets of binary questions derived from annual examination data is shown. The positive examination results applied were compared with the literature as a cross-checking mechanism. The examination identified as most likely to be positive was recommended prospectively in a protocol for the following year. Two of 5 years are illustrated to emphasize the development of the methodology. RESULTS: Application of this model in testing its validity, since 1990 at Madigan Army Medical Center, allows for the generation of a new protocol each year to prospectively improve clinical definition. CONCLUSIONS: A 5-year analysis of small bowel examination protocols, subdivisions, and odds ratios, will be forthcoming. The 2 years illustrated show how strongly our practice was influenced in using enteroclysis or in using the small bowel follow through as the examination of choice in the various clinical categories of small bowel disease: (1) practice was influenced by protocol and (2) outcome was steered toward positive examinations.     

New use of Foley catheter for precise vesicourethral anastomosis during radical retropubic prostatectomy

PURPOSE: We describe a new method of using a Foley catheter to assist vesicourethral anastomosis during radical retropubic prostatectomy. MATERIALS AND METHODS: A total of 81 patients underwent radical retropubic prostatectomy with this technique. Followup ranged from 4 to 48 months. Peri-catheter urethrograms were performed at 3 weeks. Patients were evaluated specifically for bladder neck contracture, urinary continence and prolonged catheterization. RESULTS: Bladder neck contracture, occurred in 4.9% of the patients and 87.6% were completely continent of urine. Only 1 patient required extended postoperative catheterization. CONCLUSIONS: Use of a Foley catheter for vesicourethral anastomosis is consistent and simple, and provided good surgical results in our experience.     

Automatic generation of explanations: AGE

Explaining how engineering devices work is important to students, engineers, and operators. In general, machine generated explanations have been produced from a particular perspective. This paper introduces a system called automatic generation of explanations (AGE) capable of generating causal, behavioral, and functional explanations of physical devices in natural language. AGE explanations can involve different user selected state variables at different abstraction levels. AGE uses a library of engineering components as building blocks. Each component is associated with a qualitative model, information about the meaning of state variables and their possible values, information about substances, and information about the different functions each component can perform. AGE uses: (i) a compositional modeling approach to construct large qualitative models, (ii) causal analysis to build a causal dependency graph, (iii) a novel qualitative simulation approach to efficiently obtain the system's behavior on large systems, and (iv) decomposition analysis to automatically divide large devices into smaller subsystems. AGE effectiveness is demonstrated with different devices that range from a simple water tank to an industrial chemical plant.     

Television, Gender, and Labor in the Global City

Situated within a critical, feminist approach to development communication, this study examines the role of television advertising among unskilled female factory laborers in Bangalore, India. Ethnographic fieldwork spanning the summers of 1997 and 2000 showed that factory labor awards the female worker a degree of autonomy and purchasing power in the short term, but denies her long-term empowerment because her labor is fragmented and made dispensable by the very nature of capitalist discipline. Through a discussion of gender, labor, and television in the global city, the analysis concludes that participatory communication and further ethnographic analyses are essential for long-lasting policy and social action.     

Within-plant variation in concentrations of amino acids,sugar, and sinigrin in phloem sap of black mustard,Brassica nigra (L.) Koch (Cruciferae)

Although within-plant variation in the nutrient and allelochemical composition of phloem sap has been invoked to explain patterns of host use by phloem-feeding insects, little is known about within-plant variation in phloem chemistry. Here I describe a new technique in which I use the green peach aphid,Myzus persicae Sulz., to investigate within-plant variation in the concentrations of chemicals in the phloem sap of black mustard,Brassica nigra (L.) Koch (Cruciferae). Relationships between the concentrations of chemicals in aphid diets and honeydew were established using honeydew from aphids fed on artificial diets with known concentrations of amino acids, sucrose, and sinigrin. These relationships were applied to honeydew from aphids fed on different aged leaves of black mustard to estimate the concentrations of the chemicals in phloem sap. Sinigrin concentration was estimated to be high (>10 mM) in phloem sap in young leaves, calling into question the prevailing opinion that phloem sap contains only low concentrations of allelochemicals. High concentrations may function as defenses against sap-feeding herbivores. Within-plant variation in phloem sap composition was high: (1) young leaves had high concentrations of nutrients (216 mM amino acids, 26% sugar) and sinigrin (>10 mM); (2) mature and presenescent leaves had lower concentrations of nutrients (77–83 mM amino acids, 19–20% sugar) and low concentrations of sinigrin (1–2 mM); and (3) senescing leaves had high concentrations of nutrients (199 mM amino acids, 25% sugar) and low concentrations of sinigrin (3 mM).     

Analysis of Distance Collaboration Modalities: Alternatives to Meeting Face-to-Face

Physical and temporal boundaries are rapidly diminishing in an age of swiftly advancing information communication technologies. Today’s technologically sophisticated business and organizational environments offer a number of distance collaboration modalities (DSMs) that can facilitate efficient and cost-effective meetings that have traditionally required face-to-face meeting venues. Selection of the DCMs that maximize meeting effectiveness is significantly challenging given the wide diversity of available options. Theory and empirical data can be useful as a guide in the selection of the most appropriate DCMs to maximize organizational productivity and success. This article reviews the literature on the behavioral and technical affordances and limitations of the various DCMs. It also provides a theoretical model based on media synchronicity theory to help guide selection of effective DCMs. This theoretical framework emphasizes the primacy of media alignment with core communication processes as well as social and interpersonal factors that influence meeting effectiveness.     

PROTOTYPICAL CASES FOR RETRIEVAL, REUSE, AND KNOWLEDGE MAINTENANCE IN BIOMEDICAL CASE-BASED REASONING

Representing biomedical knowledge is an essential task in biomedical informatics intelligent systems. Case-based reasoning (CBR) holds the promise to represent contextual knowledge in a way that was not possible before with traditional knowledge-based methods. One main issue in biomedical CBR is dealing with the rate of generation of new knowledge in biomedical fields, which often makes the content of a case base partially obsolete. This article proposes to make use of the concept of prototypical case to ensure that a CBR system would keep update with current research advances in the biomedical field. Prototypical cases have served various purposes in biomedical CBR systems, among which to organize and structure the memory, to guide the retrieval as well as the reuse of cases, and to serve as bootstrapping a CBR system memory when real cases are not available in sufficient quantity and/or quality. This paper emphasizes the different roles prototypical cases can play in CBR systems, and presents knowledge maintenance as a very important novel role for these prototypical cases.